GSM3531635: 30_Day16eGFP_pos_3; Homo sapiens; RNA-Seq - SRA

SRX5180692: GSM3531635: 30_Day16eGFP_pos_3; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 13.5M spots, 2.1G bases, 761.4Mb downloads

Submitted by: NCBI (GEO)

Study: In vivo developmental trajectories of human podocyte development inform in vitro differentiation of pluripotent stem-cell derived podocytes

PRJNA511894 • SRP174499 • All experiments • All runs

show Abstracthide Abstract

To assess in vitro derived podocytes, we examined the transcriptional changes during human podocyte development and applied that knowledge to pinpoint strengths and limitations of hESC-derived podocytes. Overall design: We performed transcriptionaling profiling of kidney organoids and organoid-derived MAFB-eGFP+ podocytes at various differentiation time points.

Sample: 30_Day16eGFP_pos_3

SAMN10645043 • SRS4187574 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Ambion Recoverall Total Nucleic Acid Isolation Kit and QIAGEN Rneasy Micro Kit; Qiagen Rneasy micro kit Kapa stranded mRNA-Seq library (#KK8420)

Experiment attributes:

GEO Accession: GSM3531635

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 13.5M spots, 2.1G bases, 761.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR8370605	13,519,308	2.1G	761.4Mb	2019-06-20

ID:: 6995760

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